Pseudomonas protegens CHA0 life-history traits

2020-06-21T06:42:52Z (GMT) by Erqin Li
We allowed the rhizosphere bacterium Pseudomonas protegens CHA0 to evolve on the roots of Arabidopsis thaliana for six plant growth cycles. To quantify changes in bacterial phenotypes, sixteen evolved bacterial colonies were randomly selected from each of the five plant replicate selection lines at the end of the second, fourth and sixth growth cycle, in addition to sixteen randomly selected ancestral colonies (in total 256 isolates). Then k-means analysis was applied to determining novel bacterial phenotypes. A subset of isolates of each bacterial phenotypes was characterised for their effects on plant root architecture and above and belowground biomass in separate assays at the end of the selection experiment. Supplementary dataset 1: Sheet 1: Summary table for the set of fourteen phenotypic traits for the 256 characterised isolates used for K-mean clustering. Sheet 2: Carbon use data for the 256 characterised isolates. In both sheets, each line corresponds to one isolate and each column to one specific trait. See material and methods for a detailled description of experimental procedures. Supplementary dataset 2: Sheet 1: Recapitulation of the origin (replicate line), time point and phenotype of each of the 30 isolates tested in details for their interactions with the host plant. Sheet 2: Summary table for interactions between each of the 30 isolates tested for plant growth promotion or inhibition, including effect on plant performance and bacterial density in the rhizosphere. Sheet 3: Scopelaetin sensitivity and GUS expression. See material and methods for a detailled description of experimental procedures.




CC BY 4.0