Protein ID-Mass spec_OCT4 interacting protein in neuroblastoma cells

2019-07-19T09:09:55Z (GMT) by Min Kang
OCT4-mycDDK recombinant protein was expressed in a human neuroblastoma cell line. OCT4-mycDDK protein was pulled down by immunoprecipitation (IP) using EZview Red anti-FLAG M2 affinity purification, and the protein was separated on SDS-PAGE to cut the appropriate bands out to be analyzed at the Proteomics Core Facility of University of Texas Southwestern Medical Center, Dallas, TX