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Detection of Salivary Protein Biomarkers in a Primary Sjögren Syndrome Murine Model

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posted on 19.07.2019 by Qian Tao, Peisheng Liang
Salivary biomarkers related to saliva secretion disorder were identified in a primary Sjögren syndrome (pSS)pSS murine model, NOD/ShiLtJ mouse, using differential proteomic analysis. We used 14-week-old female NOD/ShiLtJ mice and age- and sex-matched BALB/c mice as controls. 30 stimulated saliva samples and mixed them into six protein pools (three pSS pools and three control pools) for differential proteomic analysis.After Nano-high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) separation, 98.14% (69,276/69,276) spectra were used for protein identification. We identified 1,101 proteins from saliva; 68.12% (750/1,101) were detected both in NOD/ShiLtJ and BALB/c mice .Two mice strains showed a similar salivary protein spectrum. Differentially expressed proteins were determined by applying Mann-Whitney test, corrected by Benjamini-Hochberg method at a significance level of p<0.03173 and fold change of >1.5.

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