Boysen et al. Supplemental Figure 4
datasetposted on 22.07.2019 by Matthias Mayer
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Boysen et al. "Hsp70 and Hsp90-mediated Regulation of the Conformation of p53 DNA binding domain and p53 Cancer Variants", Supplemental Figure S4 related to figure 2: p53-DBD wild type and mutant proteins remain soluble when pre-incubated at 37°C in the presence of Hsp70 and Hdj1. In the absence of chaperones (left panel) or in the presence of Hsp70, Hdj1 and ATP (right panel) p53-DBD-wt was pre-incubated for 70 min at 37°C, p53-DBD-R175H 10 min at 37°C, p53-DBD-R248Q and p53-DBD-R249S for 30 min at 37°C. Subsequently, aggregated and soluble (S) fractions were separated by centrifugation and supernatant (S) and pellet (P) were analyzed by SDS-polyacrylamid gel electrophoresis and Coomassie Brillant Blue staining.